洞庭湖区双季超级杂交稻栽培示范

湘阴县2008年示范种植696．7hm^2双季超级杂交稻，取得了显著的增产、增收效果，详细介绍了品种搭配、浸种催芽、播期安排、培育壮秧、大田耕整、施肥、灌溉、病虫防治等配套栽培技术以及存在的问题，并提出了建议。     

银染mRNA差异显示方法在致突变实验中的初步应用

为建立在药物致突变研究中应用银染 m RNA差异显示的方法 ,试验提取未经过 /经过环磷酰胺处理的 Balb/c小鼠肝组织的总 RNA ,并以此为模板 ,采用 d T1 2 CG、d T1 2 AG、d T1 2 GG为锚定引物 ,通过反转录、差异显示 PCR反应 ,以 6 %变性聚丙烯酰胺凝胶电泳分离差异条带 ,回收后将其再扩增。结果表明 ,RNA投入量为 3μg、镁离子浓度为 1.5～ 2 .0 mm ol/L、退火温度为 4 0℃时 ,扩增的片段条带清晰、背景低 ,差异条带明显 ,再扩增条带单一。银染 m RNA差异显示技术可成功应用于药物致突变的研究。     

Islet cells modified with PD-L1-GPI molecule inhibit attack of reactive T cells

AIM: To investigate the immune depressive effect on the reactive T cells and to explore the immunologic injury mechanism of beta cells of islet in type 1 diabetes mellitus (DM-1). METHODS: pAd5/ PD-L1-GPI adenovirus vector with target gene was constructed and transfected into NIT cells which are known as a mouse insuloma cell line. The highly expressed membrane protein of PD-L1-GPI was confirmed by Western blotting. The peripheral blood non-adherence lymph leukocytes and target cells were cultured to detect lymph leukocyte proliferation and the T cell function. The level of IL-2, TNF-α and IFN-γ were detected in the cell culture fluid. RESULTS: Compared with the control group, the NIT cells modified with PD-L1-GPI inhibited the sensitized lymph leukocyte proliferation effectively and down-regulated the level of some cytokine secretions such as IL-2, IFN-γ and TNF-α (P<0.05). CONCLUSION: The islet cells expressed the PD-L1 gene inhibit the reactive responsive T cells, block up the cytotoxic effect of autoimmunity T cells, and induce the immunotolerance, which would be a value direction of the therapy of DM.     

Effect of angiontensin-(1-7) on development of monocrotaline induced pulmonary arterial hypertension and vascular remodeling

AIM: To investigate the effect of angiotensin-(1-7) ［Ang-(1-7)］ on the development of monocrotaline (MCT) induced pulmonary arterial hypertension and vascular remodeling.METHODS: 60 Sprgue-Dawely rats were randomly assigned into three groups: control group, PAH group and PAH+Ang-(1-7) group. Rats in PAH group and PAH +Ang-(1-7) group received 60 mg/kg MCT injection subcutaneously and after 24 h received either saline or 24 μg·kg-1·h-1 of Ang-(1-7) injection via osmotic minipumps for 4 weeks. These rats in control group were firstly injected saline subcutaneously and then received saline injection via osmotic minipumps.RESULTS: After 4 weeks, in PAH group, right ventricular systolic pressure (RVSP), right ventricular hypertrophy index (RVHI), percentage of wall thickness (WT%) and percentage of wall area (WA%) of pulmonary artery were significantly increased and NO concentration, the level of endothelial nitric oxide synthase (eNOS), eNOS Ser1177-phosphorylation were significantly decreased compared with control group. However, RVSP, RVHI, WT %, WA % were dramatically decreased in PAH +Ang-(1-7) group and NO concentration, the level of eNOS protein, eNOS Ser1177-phosphorylation were significantly increased compared with PAH group.CONCLUSION: These results suggest that Ang-(1-7) could prevent the development of monocrotaline induced pulmonary arterial hypertension and vascular remodeling, which appears to be associated with up-regulation of NO concentration and the level of eNOS protein, eNOS Ser1177-phosphorylation.     

褐色凝固型酸奶的制备工艺研究

褐色酸奶是以牛乳和葡萄糖为主要原料,通过加热产生美拉德反应,对褐变后的牛乳进行接种和发酵,得到褐色酸奶制品。本研究以感官得分和乳清析出率为指标,通过单因素试验及正交试验,研究并确定了褐色酸奶的加工工艺参数。试验结果表明,褐色酸奶的最佳加工工艺参数为葡萄糖添加量6%,褐变温度95 ℃,褐变时间5 h,发酵剂接种量5%,果胶添加量0.2%,发酵时间6 h,发酵温度42 ℃。在此条件下得到的褐色酸奶感官评定分数为93分,乳清析出率为5.3%。     

四种杀虫剂对橡胶盔蚧的防效试验

2004年8月进行4种杀虫剂对橡胶盔蚧的防效试验,结果表明40%杀扑磷乳油800倍液、2.5%杀蚧乳油 800 倍液、2.5% 蛛八蚧乳油 800～1　000 倍液、40% 氧化乐果乳油 800 倍液对橡胶盔蚧 1、2 龄若虫效果显著,14d 的防效均在91.3%以上;4种药剂的 800倍液对3龄若虫、成虫的试验结果,40%杀扑磷施药后 14d 防效为 64.7%,对成虫无效;对橡胶树无药害。     

乌药种子性状产地表型变异研究

对我国乌药自然分布区14个产地采集的乌药种子进行取样观测,分别测定其种子的长、宽、重,并进行播种试验,观测种子的出苗率;分析了乌药种子形态的产地差异、地理变异规律及种子形态对种子出苗率和苗木生长的影响.分析表明:乌药种子形态存在显著的产地差异,种子的大小存在一定的经向变异,种形指数(宽)与经度呈显著的负相关.种形指数与苗期生长有密切的关系.     

四川省天然林保护和退耕还林工程若干问题的思考

四川省实施退耕还林和天然林保护工程以来，已取得了初步成效。文章简述了四川省天然林资源保护工程和退耕还林工程实施以来出现的新条件，总结了两大工程实施过程中的困难和问题，指出了解决问题的主要途径。     

新疆森林公园网站设计

森林公园是新疆的重要资源之一。本文就如何利用现代化的网络技术对新疆森林公园资源整合、共享进行初步的探索,为更加有效地利用现有的森林公园提供一个有效的途径。     

硫素的营养诊断及对土壤、环境的影响

本文对硫素的营养诊断及硫肥对土壤、环境的影响作了系统的综述。内容主要包括硫肥的种类和性质、作物硫素营养诊断方法、硫肥的营养作用、土壤中硫的形态和作用以及硫肥对大气、水体的危害,并提出了施用硫肥时应注意的问题。